Gene Pulser Xcell Eukaryotic System from Bio-Rad

Product Specs
ItemGene Pulser Xcell Eukaryotic System
CompanyBio-Rad
Price Get Pricing Get Quote for this Product
Catalog Number1652661
Brand/TrademarkBio-Rad Laboratories, Life Science Group
QuantityEA
ApplicationsElectroporation of mammalian cells and plant protoplasts
Dimensions(WxDxH) 31 x 28 x 9 cm (12.2 x 11.02 x 3.54 inches)
Voltage10 to 3000 V
Cell TypeEukaryotic cells
Weight6.6 kg
MethodElectroporation

Specifications/Features

Pulse Voltage:
10–3,000 V

Capacitance:
10–500 V, 25–3,275 degrees F in 25 degrees F increments
500–3,000 V, 10, 25, 50 degrees F

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

Website: Bio-Rad Life Science Research Product Promotions

(1) The H2Bub1-deposition complex is required for human and mouse cardiogenesisDevelopmentDecember 1, 2023Syndi Barish, Kathryn Berg, Jeffrey Drozd, Isabella Berglund-Brown, Labeeqa Khizir, Lauren K. Wasson, Christine E. Seidman, Jonathan G. Seidman, Sidi Chen, Martina Bruecknercreated by adding 10 µg of Cas9 plasmid and 10 µg of sgRNA plasmid to 1 million cells using the Bio-Rad Gene Pulser XCell Eukaryotic System with a homemade nucleofection solution (5 mM KCl, 5 mM MgCl2, 15 mM Hepes, 102.94 mM Na2HPO4 and 47.06

(2) Pathogenic Aβ production by heterozygous PSEN1 mutations is intrinsic to the mutant protein and not mediated by conformational hindrance of wild-type PSEN1Journal of Biological ChemistryAugust 1, 2023Vanessa Kurth, Isabella Ogorek, Carolina Münch, Javier Lopez-Rios, Solenne Ousson, Sandra Lehmann, Katja Nieweg, Anton J.M. Roebroek, Claus U. Pietrzik, Dirk Beher, et al.pDREV1-PSEN1 and the pDIRE vectors were added, and the cells were electroporated in a 0.4 cm cuvette using a Gene Pulser Xcell Eukaryotic Electroporation System (BIO-RAD) with the following parameters: 0.24 kV, 475 μF, one pulse ∼ 8 ms. After

(3) A single-cell massively parallel reporter assay detects cell-type-specific gene regulationNature GeneticsFebruary 1, 2023Siqi Zhao, Clarice K. Y. Hong, Connie A. Myers, David M. Granas, Michael A. White, Joseph C. Corbo, Barak A. Cohenplasmid was transformed using Gene Pulser Xcell Electroporation Systems by electroporation (Bio-Rad 1652661) into 50μl of ElectroMax DH10B electrocompetent cells (Invitrogen 18290015) with 1μl of assembled product

(4) Mechanism of IFT-A polymerization into trains for ciliary transportCellDecember 1, 2022Shimi Meleppattu, Haixia Zhou, Jin Dai, Miao Gui, Alan Brownimmediately transferred to a pre-cooled electroporation cuvette (d=2 mm) and electroporated using a Gene Pulser Xcell Eukaryotic System (Bio-Rad) using time constant protocol at 450 V for 3.5 ms. The cuvette was then put back on ice for

(5) Contaminating transfection complexes can masquerade as small extracellular vesicles and impair their delivery of RNAJournal of extracellular vesiclesOctober 1, 2022McCann J, Sosa-Miranda CD, Guo H, Reshke R, Savard A, Zardini Buzatto A, Taylor JA, Li L, Gibbings DJ.resuspended in Opti‐MEM prior to electroporation with a Gene Pulser Xcell Electroporation System (Bio‐Rad, 1652661). The preprogrammed protocol for transfection of HEK 293 in a 2 mm cuvette was followed. However, voltage

(6) Toxoplasma gondii Rhoptry Protein 7 (ROP7) Interacts with NLRP3 and Promotes Inflammasome Hyperactivation in THP-1-Derived MacrophagesCellsMay 12, 2022Zhu L, Qi W, Yang G, Yang Y, Wang Y, Zheng L, Fu Y, Cheng X.tachyzoites in a final volume of 400-μL Cytomix Buffer through the Gene Pulser Xcell Eukaryotic System (#1652661, Bio-Rad, Hercules, CA, USA). The electroporation parameters were 1.7 kV for two 176-μs pulses in 5-s

(7) Efficient targeting of NY-ESO-1 tumor antigen to human cDC1s by lymphotactin results in cross-presentation and antigen-specific T cell expansionJournal for ImmunoTherapy of CancerApril 15, 2022Camille Le Gall et al.electroporation cuvette (1652088, BioRad), and transfected using a Gene Pulser Xcell electroporation system (1652661, BioRad) using the following settings: square wave, 500 V, 3 ms, 1 pulse, 4 mm. Cells were carefully

(8) A transgenic mouse embryonic stem cell line for puromycin selection of V0(V) interneurons from heterogenous induced culturesStem cell research & therapyMarch 28, 2022Pardieck J, Harb M, Sakiyama-Elbert SE.electroporation cuvette (Bio-Rad, 1652081), mounted into the Gene Pulser Xcell Eukaryotic System (Bio-Rad, 1652661), and electroporated with voltage parameter 0.23 kV and capacitance parameter 960 µF. After pulsing,

(9) Systematic optimization of square-wave electroporation conditions for bovine primary fibroblastsBMC Molecular and Cell BiologyDecember 1, 2020Iqbal Hyder, Shahin Eghbalsaied, Wilfried A. Kues(Cell Projects, #EP-104). A BioRad Gene Pulser Xcell (Gene Pulser Xcell Eukaryotic System, Order no:1652661) was used for pulsing, the following parameters could be set for square wave pulses: voltage, pulse

(10) A puromycin selectable cell line for the enrichment of mouse embryonic stem cell-derived V3 interneuronsStem Cell Research & TherapyDecember 1, 2015Hao Xu, Nisha Iyer, James E. Huettner, Shelly E. Sakiyama-Elbertoriginally gifted by Keith Joung and Jin-Soo Kim, respectively. Cells were electroporated using a Biorad Gene Pulser Xcell Eukaryotic System at 0.23 kV and 975 μF in a 0.4 cm cuvette (Bio-Rad, Hercules, CA). Following electroporation, cells

(11) The Replacement of 10 Non-Conserved Residues in the Core Protein of JFH-1 Hepatitis C Virus Improves Its Assembly and SecretionPLOS ONESeptember 4, 2015Loïc Etienne, Emmanuelle Blanchard, Audrey Boyer, Virginie Desvignes, Julien Gaillard, Jean-Christophe Meunier, Philippe Roingeard, Christophe Houriouxcells with 5 μg of recombinant SFV RNA and electroporated them by a single pulse at 350 V, 750 μF (Gene Pulser Xcell™ Eukaryotic System, Biorad). The electroporated cells were then diluted in growth medium, plated in 75 cm2 culture dishes,

(12) Reduced mtDNA copy number increases the sensitivity of tumor cells to chemotherapeutic drugsCell Death & DiseaseApril 2, 2015H Mei, S Sun, Y Bai, Y Chen, R Chai, H Li-cm sterile electroporation cuvette with DMEM containing no antibiotics and were transfected with a Gene Pulser Xcell Eukaryotic System (Bio-Rad, Hercules, CA, USA) at 250 V and a capacitance of 950 μF. Preparation of the mtDNA probe and

(13) Variations in PROKR2, but not PROK2, are associated with hypopituitarism and septo-optic dysplasiaThe Journal of Clinical Endocrinology & MetabolismMarch 1, 2013Mark J. McCabe, Carles Gaston-Massuet, Louise C. Gregory, Kyriaki S. Alatzoglou, Vaitsa Tziaferi, Oualid Sbai, Philippe Rondard, Koh-hei Masumoto, Mamoru Nagano, Yasufumi Shigeyoshi, et al.cultivated in DMEM supplemented with 10% fetal calf serum and transfected by electroporation with a Gene Pulser Xcell eukaryotic system (Bio-Rad, Hercules, California) as described previously (13). Then, 107 cells were transfected with

(14) Urodele p53 tolerates amino acid changes found in p53 variants linked to human cancerBMC Evolutionary BiologyDecember 1, 2007Éric Villiard, Henner Brinkmann, Olga Moiseeva, Frédérick A Mallette, Gerardo Ferbeyre, Stéphane Roypromoter (1 μg) was used to normalize the results of the assays. The transfection was performed using a Gene-Pulser Xcell Eukaryotic System (Bio-Rad) generating a single electrosquare pulse of 250 volts during 35 milliseconds, in a 4 mm cuvette

(15) Human Ccr4-Not complex is a ligand-dependent repressor of nuclear receptor-mediated transcriptionThe EMBO JournalJuly 12, 2006G Sebastiaan Winkler, Klaas W Mulder, Vivian J Bardwell, Eric Kalkhoven, H Th Marc Timmerscuvette (0.1 cm gap, BioRad) and subjected to 16 pulses (1.5 ms, 1.5 s intervals) at 140 V using a Gene Pulser Xcell eukaryotic system (BioRad). Cells were resuspended in 4 ml medium, and plated in four wells of a 12-well plate. The next day,

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